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Clinical Studies
Abstracts are presented below for clinical
studies on Malabar Nut Tree.
Plant Phytonutrient Profile
1: Mol Cell Biochem. 2000 Oct;213(1/2):99/109.
Modulatory influence of Adhatoda vesica (Justicia adhatoda) leaf extract on the
enzymes of xenobiotic metabolism, antioxidant status and lipid peroxidation in
mice.
Singh RP, Padmavathi B, Rao AR.
Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University,
New Delhi, India.
The effect of two different doses (50 and 100 mg/kg body wt/day for 14 days) of
80% ethanolic extract of the leaves of Adhatoda vesica were examined on drug
metabolizing phase I and phase II enzymes, antioxidant enzymes, glutathione
content, lactate dehydrogenase and lipid peroxidation in the liver of 8 weeks
old
Swiss albino mice. The modulatory effect of the extract was also examined on
extra/hepatic organs viz. lung, kidney and forestomach for the activities of
glutathione S/transferase, DT/diaphorase, superoxide dismutase and catalase.
Significant increase in the activities of acid soluble sulfhydryl (/SH) content,
cytochrome P450, NADPH/cytochrome P450 reductase, cytochrome b5, NADH/cytochrome
b5 reductase, glutathione S/transferase (GST), DT/diaphorase (DTD), superoxide
dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione
reductase (GR) were observed in the liver at both dose levels of treatments.
Adhatoda vesica acted as bifunctional inducer since it induced both phase I and
phase II enzyme systems. Both the treated groups showed significant decrease in
malondialdehyde (MDA) formation in liver, suggesting its role in protection
against prooxidant induced membrane damage. The cytosolic protein was
significantly inhibited at both the dose levels of treatment indicating the
possibility of its involvement in the inhibition of protein synthesis. BHA has
significantly induced the activities of GR and GSH in the present study. The
extract was effective in inducing GST and DTD in lung and forestomach, and SOD
and CAT in kidney. Thus, besides liver, other organs viz., lung, kidney and
forestomach were also stimulated by Adhatoda, to increase the potential of the
machinery associated with the detoxification of xenobiotic compounds. But, liver
and lung showed a more consistent induction. Since the study of induction of the
phase I and phase II enzymes is considered to be a reliable marker for
evaluating
the chemopreventive efficacy of a particular compound, these findings are
suggestive of the possible chemopreventive role played by Adhatoda leaf extract.
Publication Types:
Research Support, Non/U.S. Gov't
PMID: 11129964 [PubMed / indexed for MEDLINE]
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