Clinical Studies

Abstracts are presented below for clinical studies on Yellow-Fruit Nightshade.

• Botanical Name: Solanum Virginiana

• Ayurvedic Name: Kantakari

• Common Name: Yellow-Fruit Nightshade

Plant Phytonutrient Profile

1: J Plant Physiol. 2006 Mar;163(5):546/​56. Epub 2005 Aug 1.

A novel pathogenesis/​related protein (SsPR10) from Solanum surattense with
ribonucleolytic and antimicrobial activity is stress/​ and pathogen/​inducible.

Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun X, Tang K.

State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan/​Tan
International Center for Life Sciences, Fudan/​SJTU/​Nottingham Plant Biotechnology
R&D Center, Fudan University, Shanghai 200433, PR China.

A cDNA clone (designated as SsPR10, GenBank Accession Number AY660753 ) encoding
a PR10 protein from yellow/​fruit nightshade (Solanum surattense) was isolated and
characterized. SsPR10 encoded a 160/​amino/​acid polypeptide with a predicted
molecular mass of 17.58 kDa and pI of 5.29. Sequence alignments showed that
SsPR10 had high identity (68.1%) with CaPR10, but had only about 31.7% identity
with JIOsPR10 at the amino acid level. Genomic DNA gel blot analysis indicated
that SsPR10 belonged to a multigene family. The constitutively expressed SsPR10
was detected to be the highest in roots of the sterile seedlings cultured in
jars, while SsPR10 expression was the highest in old yellow leaves from the
seedlings incubated with sap containing TMV. SsPR10 always expressed at slightly
higher level in senescent leaves than in tender ones under both conditions.
Further expression analysis revealed that the signaling components of
defense/stress pathways (MeJA, SA, ABA, GA3, H2O2 and Cu2+) up/​regulated
significantly the SsPR10 mRNA levels over the control. However, darkness failed
to induce SsPR10 expression and its expression was also inhibited by cold
treatment. The SsPR10 was successfully expressed in Eschericha coli and the
expressed protein was purified to near homogeneity. The dialytically renatured
SsPR10 protein without phosphorylation exhibited ribonucleolytic activity against
S. surattense leaf total RNA preparations and could inhibit hyphal growth of
Pyricularia oryzae. Our findings suggest that the novel stress/​ and
pathogen/​inducible SsPR10 with ribonucleolytic and antimicrobial activity
participates not only in the defense/stress response pathways but also in plants'
growth, development and senescence.

Publication Types:
Research Support, Non/​U.S. Gov't

PMID: 16473659 [PubMed /​ indexed for MEDLINE]

2: Mol Biol (Mosk). 2005 Sep/​Oct;39(5):786/​98.

[Isolation and expression profiling of the Pto/​like gene SsPto from Solanum
surattense]

[Article in Russian]

Huang B, Liu X, Wang X, Pi Y, Lin J, Fei J, Sun X, Tang K.

A novel Pto/​like gene (designated as SsPto) is cloned from yellow/​fruit
nightshade (Solanum surattense). The full/​length cDNA of SsPto is 1331 bp long
with an open reading frame of 960 bp encoding a polypeptide of 320 amino acid
residues. The deduced SsPto protein has a calculated molecular weight of 36.21
kDa with an isoelectric point of 6.18. Multiple sequence alignment shows that
SsPto protein shares 71.4% and 71.6% identities to Pto proteins from Lycopersicon
pimpinellifolium and L. hirsutum respectively. Genomic Southern blot analysis
indicates the presence of a small family of SsPto in the S. surattense genome.
SsPto is found to be constitutively expressed in the S. surattense plant with the
highest expression in stems. However, under induction by TMV for 6 days, SsPto
expresses the highest in roots. Further expression analysis reveals that the
signaling components of defense/stress pathways, such as methyl jasmonate (MeJA),
salicylic acid (SA), gibberellic acid (GA3) and hydrogen peroxide (H2O2),
up/​regulate the SsPto transcript levels over the control. Cold treatment,
nevertheless, has no significant effect on SsPto expression whereas SsPto
expression is down/​regulated by dark treatment. Our findings suggest that this
novel stress/​ and pathogen/​inducible SsPto from S. surattense may participate not
only in the defense/stress responsive pathways, but also in diverse processes of
plant's growth and development.

Publication Types:
English Abstract

PMID: 16240712 [PubMed /​ indexed for MEDLINE]